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Immune-Focused Library Construction and Screening
Generating bespoke antibody library focused on your specific target
Overview

Immune-focused library is generated using B cells of various animal species which have immunized with the specific antigen. Immune-focused library construction and screening service is designed to isolate specific and high affinity antibodies against the target of interest compared with the antibodies discovered from the naïve antibody library.

Y-Biologics offers the construction of the immune-focused library in the form of a phage display library by using cDNA extracted from mouse spleens and suggests antibody screening service with optimized panning methods against the target to discover antibodies with high affinity. We also propose antibody optimization(See more) including antibody maturation and humanization if clients would like to develop the antibodies generated from immune-focused library as a therapeutic purpose to reduce the immunogenicity and enhance the binding affinity.

Difference between Naïve library and Immune-focused library
Naïve library (Ymax®-ABL)
Immune-focused library
Species
Human
Mouse
Applied antigen for screening

Universal

One

Diversity

Huge
Medium
Timelines
11 weeks
8-12 weeks (Immunization of mice) + 17 weeks
Animal use
No
Yes
Potential immunogenicity
Low
High
Affinity of binders
Medium
High
Humanization
No need
Need
Price
+++
++
Timeline of Immune-Focused Library Construction and Screening
8~12 weeks
Immunization of mice (Outsourcing)
6 weeks
cDNA synthesis & Construction of Library
4 weeks
Bio panning
4 weeks
Screening
1 week
Sequencing
2 weeks
Final Report
Customer preparation: Protein(s) with different tags
Deliverables: Final report, Antibody variable region sequences
  • There may be changes depending on the conditions of the experiment.
  • Details can be coordinated at each stage, so please contact us for more information.
Points of Differentiation
High Affinity
Antibodies with
higher binding affinity
More possibility
Increase the possibilities of discovering antibodies with diverse sequences by utilizing two types of libraries
Neutralizing antibodies
More optimized method for the generation of neutralizing antibody
Online Inquiry
There is an additional discount promotion if you request the immune-focused library
construction and screening with our related service, Antibody Humanization. Click here to learn details